This study was carried out at Rashad and Alabassia localities, South Kordofan, Sudan. The objective of the study is to assess the impact of elevation on species richness and density. To understand the dynamic trends of the vegetation across the studied area, the compositional and structural features of three plant layers (mature tree and shrubs, regeneration and herbaceous) at 14 investigation sites were compared, in terms of species richness, species evenness and density. Shannon Biodiversity index (H), Margalef index (Species richness (D) and Pielou index for species evenness (E) were calculated. Results of ecological indices show that their values in the mature trees and shrubs and regeneration layers increase with increment of elevation, while they decrease with increment of elevation in herbaceous layer, the density of mature trees and shrub and regeneration layers decreases with increment of elevation and increases with increment of elevation in herbaceous layer. More research is needed to assess the altitudinal effect in the natural vegetation in the other Localities of the Nuba Mountains.