University Publications

This work focuses on isolating the indigenous microorganisms in petroleum contaminated sites and investigating their efficiency to degrade the crude oil of Heglig oil field in Sudan. The selected isolates HP, HWH and HAC (which were identified as Bacillus safensis, Bacillus pumilus and Actinomycetes) were studied for 90 days to assess their ability to degrade crude oil (1%, 3%, 5% and 10%) individually and in consortia. The potential isolates HP, HWH and HAC were identified as Bacillus saefnsis, B. pumplis and Actinomyses, respectively. The ability of consortium isolates to degrade crude oil was increased. The biodegradation of consortium isolates HP+HWH comparing with HP as individual of 3 % crude oil from 56.1% to 90.02%, and of 5% crude oil from 38.7% to 64.5% respectively. The consortium of HP+HWH was the best among the other consortia in Heglig isolates. This consortium was followed by the consortium of three isolates HAC+HP+HWH. All consortia which included isolate HAC (HAC+HP, HAC+HWH and HAC+HP+HWH) showed low efficiency compared to individual isolates.