University Publications

This work was carried out to study the competence of coliphages (isolated from Soba Stabilization Station, from the Anaerobic, Facultative, and Maturation ponds) against E. coli strains using the chloroform technique and quantified using UV-visible spectrophotometer and the double layer agar technique for plaque assay. The interaction between bacterial cultures and isolated phages was monitored using a UV-vis spectrophotometer, the optical density at OD600 was monitored at two stages of infection; the first infection, where it ranges between 0.405–0.686 AU, and the second infection, which gives rise to a range of optical density between 0.233–0.250 AU. In addition to the OD600 measurements, double layer agar technique showed variations in OD per pond and sampling site. Inhibition activities of the isolated coliphages were performed by the method of Miles and Misra (1938). The phages showed variation in inhibition activities with best result found on Luria Bertani (LB) semi solid medium.