University Publications

There are numerous plants, which contain some compounds in its chemical constituents which have inhibitory effects on microbial growth, specially, the pathogenic bacteria and fungi. Aloe vera is of Aloaceae family growing in eastern and northern Africa. It was chosen for this study because of its uses as an old remedy in traditional medicine for treatment of wound and second degree burns, and in recent uses form treatment of some infections. This research aims to analysis and determination of secondary metabolism of Aloe vera leaf and sap extracts using different chromatographic methods. The fine powder of the A. vera leaf and sap was extracted with several solvents (methanol, ethanol, acetone and water) and the anti– microbial effect on of each extract was evaluated and the methanol extract showed high activity. A phytochemical investigation was also carried out on ethanol and aqueous extracts of A. vera (leaf and sap) which represented the presence of tannins, acids, alkaloids, steroids, flavonids, saponins, triterpenes, and coumarins. The water represented 95.6% of total weight of A. vera leaves. Intensive analytical investigations were carried out on the most active methanolic extract in order to isolate and identify the effective components using column and preparative thin layer chromatography. Five pure compounds isolated from fractions sap, two pure compounds isolated from leaf and were analyzed by Infra-red spectroscopy and Ultra-violet spectroscopy.