University Publications

Background: Recently, the green synthesis of metal nanoparticles has gained much interest and there is an increasing demand for various nanoparticles due to their extensive applicability in various areas such as clinical and medical applications, consumer products, electronics and equipment, catalysis, chemistry, energy and gaining more importance due to its simplicity, non-toxic an environmental friendly, cost effective, easy process. Objective: This study investigate an efficient, sustainable route and eco-friendly technique for green synthesis of titanium nanoparticles using fruits of Tribulus terrestris, for their wide availability and medicinal property, and to evaluate the toxicity of different doses Ti-NPs on Wister Rats. Methods: TiNPs were prepared by the reaction of 0.5M titanium tetrachloride and 20% fruits extract of Tribulus terrestris (Dirassa). The TiNPs were characterized by x-ray diffraction (XRD),scanning electron microscopy (SEM), Energy dispersive X-Ray Spectroscopy (EDS), UV-Visible spectroscopy (UV) and tested for their toxicity on Wister rats .The extracts was prepared and given to rats orally once a day for 8 weeks. Body weight was measured weekly. Biochemical effects (serology and hematology) and Histopathological studies of liver, kidney, intestine, brain and heart were performed. Results: Nanoparticles were characterized. The size of the obtained TiNPs with size 6 nm and the shapes is homogenous under the preparation conditions. Body weight after 8weeks of treatment of rats with Ti nanoparticles was significantly higher in treated groups when compared with control animals (p <0.05), the hematology show significant (P <0.05) decrease in MCHC, MCH and Lymphocytes and increase in neutrophils. ALP and creatinine was significant (P <0.05) decrease in all treated groups .The results of histopathology examination from various treatment groups revealed that the damage in high dose and showed less damage and low index of changes in medium dose and no any change or damage to the vital organs in lower dose.