University Publications

Background: Green synthesis of nanoparticles is becoming one of the strong techniques, which may be suitable alternatives for chemical and physical method and had many uses in optical, electrical, and thermal properties and almost all types of industries from textiles to medicine. Biosynthesis of nanoparticles is a bottom up approach where the main reaction occurring is reduction/oxidation by plant. Objective: This study was carried out to evaluate the histological and biological activities in Reproductive System of Titanium, Tribulus terrestris Titanium nanoparticles (Tt-Ti-NPs) and aqueous extract of Tribulus terrestris fruits(Dirassa) on Wistar rats. Methods: Dirassa extract was used for the biosynthesis of TiNPs. TiNPs was prepared and characterized by use UV-Visible spectroscopy (UV), x-ray diffraction (XRD), Energy dispersive X-Ray Spectroscopy (EDS), scanning electron microscopy (SEM) to determine different parameters such as particle size, shape, crystallinity, fractal dimensions, pore size and surface area and then tested for their effect on Wister rats to estimate the effect of TiNPs on reproductive system. Thirty Wister rats was divided to five groups, group one as control, group two T. terrestris fruits extract TiTtNPs, group three 50mg/kg/day (low dose), group fourTiTt NPs 300mg/kg/day (high dose) and the last group five TiNPs 300 mg/kg/day given to rats respectively oral for 30 days. Body weight was measured weekly. Sexual hormones (Testosterone, Estrogen Progesterone, LH, FSH and Prolactin), Histopathological studies of testes and ovaries were performed. Results: Nanoparticles were characterized and spherical shaped and had the average size 6 nm. After 2 weeks there was significantly change in body weight, lowering weight in treated groups when compared with control animals (p < 0.05) and after 4 weeks the weight is increase. In hormonal result for male show decrease in FSH, LH in all treated group and decrease in testosterone except in group 5. About female there was increase in progesterone in group treated with green TiTtNPs and decrease in LH and FSH. Marked reduction of reproductive hormones was observed in both male and female rats when given Titanium nanoparticles. Degeneration of the seminiferous epithelium as well as reduction of produced sperms in males, Lack of normal antral follicles, enlarge cystic follicles and presence of atretic follicles in females. Vaculation and fibrosis in some cells in rats.