<p><strong>Background: </strong>Ceftriaxone induced autoimmune hemolytic anemia (AIHA), leading to the destruction of red blood cells causing secondary anemia. The current study hemolytic anemia among Sudanese patients and explore the correlation to the duration of drug taken.</p> <p><strong>Methods:</strong> 40 samples were taken from patients treated with the ceftriaxone after surgery as antibiotic, 17 of them were females and 23 males, 10 samples from healthy persons used as control group. Direct and indirect coombs test were performed by manual method.</p> <p><strong>Results:</strong> The study results founded that the ceftriaxone drug has no effect on Sudanese patients.</p> <p><strong>Discussion: </strong>Ceftriaaxone doesn’t induce immune hemolytic anemia in our study population with p.value> 0.05 direct coombs tests indirect coombs tests were negative in all patients. Conclusion: In this study ceftriaxone was not considered as cause of drug induced immune hemolytic anemia as an adverse effect among the Sudanese patients.</p>