Background: Globally providing quality emergency obstetric care (EmOC) reduces the risk of maternal and newborn mortality and morbidity. There is evidence that over 50% of maternal health programs that result in improving access to emergency obstetric care and reduce maternal mortality have an emergency obstetric care-training component. The ability of the midwife or nurse to deal competently with the obstetric emergencies depends on the prompt action taken by her. In Sudan, more than 1 in 3 women who deliver suffer from prolonged labor, and 1 in 4 women experiences excessive bleeding. Aim: The present study aimed to assess the knowledge of nurses and midwives regarding the management of obstetrical emergency care during labor. Method: A descriptive cross-sectional, hospital based-study, the current study was conducted at specialized maternity government hospitals in Khartoum State , 81 nurses and midwives were enrolled using a total coverage technique. The data were collected by a questionnaire and were analyzed by the Statistical Packages for Social Sciences (SPSS), version 25, and then the data were presented in form of simple frequency tables and cross-tabulations to explore the relationship between variables. P-value ≤ 0.05 was considered statistically significant. Ethical approval was obtained from ethical committees and administrative authorities of hospitals, and verbal consent was obtained from the respondents enrolled in the sample of the study. Results: The findings illustrated that most(70.4%) of nurses and midwives had good knowledge about comprehensive management of emergency obstetrics during labor and half of the nurses and midwives (51.9%) had fair knowledge about initial management of an obstetric emergency during labor. Slightly above half of the study, participants (58 %) had good knowledge regarding the basic management of obstetrics emergency during labor, also there have strong significant association between level of total knowledge and training of midwifes /nurses P value 0.0 and no association between qualification and the total knowledge, P-value 0.17 Conclusion: The study concluded that most of nurses and midwives had good knowledge about comprehensive management of emergency obstetrics during labor High-quality, comprehensive emergency obstetrics care , provided primarily by nurses and midwives, has resulted in significant improvements in the availability and utilization of life-saving care at birth. Key words: Emergency obstetric care, Knowledge, Midwives, Comprehensive Management